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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1991-3-8
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pubmed:abstractText |
We adapted the ultracentrifugation method of the Lipid Research Clinics Program for the separation of lipid subfractions (LDL, VLDL and HDL cholesterol) to a tabletop ultracentrifuge (Beckman TL-100). Centrifugation time was reduced from 18 h to 2.5 h and the sample volume from 5 mL to 2 mL plasma. The imprecision of the LDL-cholesterol estimation (coefficient of variation = CV) was 2.9-7.4% and that of HDL-cholesterol measurement was 1.4-3.9%. Imprecision of the VLDL-C measurement was high (CV = 15.6-29.8%). The results correlated with those obtained by the Lipid Research Clinics method (P less than 0.001). Our method could be conveniently adapted by clinical laboratories serving specialist lipid clinics.
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pubmed:commentsCorrections | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0004-5632
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
27 ( Pt 5)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
459-64
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2281925-Cholesterol,
pubmed-meshheading:2281925-Cholesterol, HDL,
pubmed-meshheading:2281925-Humans,
pubmed-meshheading:2281925-Lipoproteins, LDL,
pubmed-meshheading:2281925-Lipoproteins, VLDL,
pubmed-meshheading:2281925-Reproducibility of Results,
pubmed-meshheading:2281925-Ultracentrifugation
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pubmed:year |
1990
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pubmed:articleTitle |
The measurement of lipoprotein subfractions in plasma using a tabletop ultracentrifuge.
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pubmed:affiliation |
Department of Pathological Biochemistry, Western Infirmary, Glasgow, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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