Linked Life Data

2280058

Source:http://linkedlifedata.com/resource/pubmed/id/2280058

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1991-3-14
pubmed:abstractText
A hemolytic method was developed for the quantitative determination of oleanane saponin (Endod-S) in Phytolacca dodecandra (Phytolaccaceae), a plant with potent molluscicidal properties. A chemical method based on thin-layer chromatography (TLC) was used as a control for comparison with a hemolysis test. Standard curves for calibration of both tests of Endod-S gave high correlation coefficients. The hemolysis method gave a correlation coefficient of 0.98 for saponin (Endod-S) levels ranging from 1.6 to 4.2 mg/l. The TLC method gave a correlation coefficient of 0.99 for saponin levels above 1.5 micrograms. Due to the action of saponins on erythrocytes, the hemolysis method could not be used at levels below 1.6 and above 4.2 mg/l. Similarly, sample size limited TLC quantitation of these saponins at levels below 1 microgram. Both the TLC and the hemolysis methods on identical Endod-S water samples gave comparable results at saponin levels between 2 and 6 mg/l. A snail bioassay showed that the LC50 and the LC90 concentrations of Endod-S to the snail Biomphalaria glabrata (albino) were 2.57 and 2.92 mg/l respectively. These values fell in the standard curve range for the hemolysis method thus indicating that the hemolysis method is of practical value for testing Endod-S concentrations that would actually be used in field applications for snail control. The hemolysis method is less expensive than TLC and can be easily adapted to field conditions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0360-1234
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
777-86
pubmed:dateRevised
2009-7-21
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
A new method for determining concentrations of Endod-S (Phytolacca dodecandra) in water during mollusciciding.
pubmed:affiliation
Dept. of Environmental Health Sciences, School of Public Health and Tropical Medicine, Tulane University Medical Center, New Orleans, LA 70112.
pubmed:publicationType
Journal Article, Comparative Study