Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12B
|
| pubmed:dateCreated |
1991-3-14
|
| pubmed:databankReference | |
| pubmed:abstractText |
HNF-4 (hepatocyte nuclear factor 4) is a protein enriched in liver extracts that binds to sites required for the transcription of the genes for transthyretin (TTR), the carrier protein in the serum for vitamin A and thyroid hormone, and for apolipoprotein CIII (apoCIII), a major constituent of chylomicrons and very low-density lipoproteins (VLDL). Synthetic oligonucleotides derived from amino acid sequence of affinity-purified HNF-4 protein (54 kD) were used in the polymerase chain reaction (PCR) to isolate a cDNA clone encoding the protein. HNF-4 is a member of the steroid hormone receptor superfamily with an unusual amino acid in the conserved "knuckle" of the first zinc finger (DGCKG). Studies with in vitro-translated HNF-4 protein show that it binds to its recognition site as a dimer, and cotransfection assays indicate that it activates transcription in a sequence-specific fashion in nonhepatic (HeLa) cells. Northern blot analysis reveals that HNF-4 mRNA is present in kidney and intestine, as well as liver, but is absent in other tissues. DNA-binding and antisera reactivity data suggest that HNF-4 could be identical to liver factor A1 (LF-A1), a DNA-binding activity implicated in the regulation of transcription of the alpha 1-antitrypsin, apolipoprotein A1, and pyruvate kinase genes. The similarity between HNF-4 and other ligand-dependent transcription factors raises the possibility that HNF-4 and the genes it regulates respond to an as yet unidentified ligand.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Steroid,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0890-9369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2353-65
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2279702-Amino Acid Sequence,
pubmed-meshheading:2279702-Animals,
pubmed-meshheading:2279702-Base Sequence,
pubmed-meshheading:2279702-Binding Sites,
pubmed-meshheading:2279702-Cell Nucleus,
pubmed-meshheading:2279702-Cloning, Molecular,
pubmed-meshheading:2279702-DNA-Binding Proteins,
pubmed-meshheading:2279702-Gene Expression,
pubmed-meshheading:2279702-Hepatocyte Nuclear Factor 4,
pubmed-meshheading:2279702-Liver,
pubmed-meshheading:2279702-Macromolecular Substances,
pubmed-meshheading:2279702-Male,
pubmed-meshheading:2279702-Molecular Sequence Data,
pubmed-meshheading:2279702-Multigene Family,
pubmed-meshheading:2279702-Oligonucleotide Probes,
pubmed-meshheading:2279702-Phosphoproteins,
pubmed-meshheading:2279702-Rats,
pubmed-meshheading:2279702-Rats, Inbred Strains,
pubmed-meshheading:2279702-Receptors, Steroid,
pubmed-meshheading:2279702-Recombinant Proteins,
pubmed-meshheading:2279702-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2279702-Transcription, Genetic,
pubmed-meshheading:2279702-Transcription Factors
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Liver-enriched transcription factor HNF-4 is a novel member of the steroid hormone receptor superfamily.
|
| pubmed:affiliation |
Laboratory of Molecular Cell Biology, Rockefeller University, New York, New York 10021.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|