|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1980-1-24
|
|
pubmed:abstractText |
Conjugates of horseradish peroxidase with antibodies (anti-human IgG (H + L)) or their Fab' fragments were prepared according to the newest modification of the periodate (P-) method or the two-step glutaraldehyde (G-) method. The conjugates were analysed by gel chromatography and subsequently tested in three different applications. For tissue immunohistochemistry on sections of lupus erythematosus skin, G-conjugates were preferred to polymeric P-conjugates. In ELISA for detection of human antibodies against penicillin P-conjugates were superior to G-conjugates. For the detection of surface Ig on lymphoid cells both types of conjugate were more or less equally suitable. A scheme for the most suitable combinations of method of preparation and field of application is given.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
0022-1759
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
30
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
245-55
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:227964-Aldehydes,
pubmed-meshheading:227964-Animals,
pubmed-meshheading:227964-Antibodies,
pubmed-meshheading:227964-Antigens, Surface,
pubmed-meshheading:227964-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:227964-Fluorescent Antibody Technique,
pubmed-meshheading:227964-Glutaral,
pubmed-meshheading:227964-Histocytochemistry,
pubmed-meshheading:227964-Horseradish Peroxidase,
pubmed-meshheading:227964-Humans,
pubmed-meshheading:227964-Immunoglobulin Fab Fragments,
pubmed-meshheading:227964-Periodic Acid,
pubmed-meshheading:227964-Rabbits
|
|
pubmed:year |
1979
|
|
pubmed:articleTitle |
Periodate or glutaraldehyde for preparing peroxidase conjugates?
|
|
pubmed:publicationType |
Journal Article,
Comparative Study
|
