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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1991-3-12
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pubmed:abstractText |
A protein complex (PC) composed of the MRP8 and MRP14 proteins has previously been shown to be a specific inhibitor of casein kinase I and II. This PC is expressed during the late stages of terminal differentiation induced in human promyelocytic HL-60 leukemia cells by 1 alpha,25-dihydroxyvitamin D3 and in human monocytic THP-1 leukemia cells by phorbol 12-myristate 13-acetate. This expression is associated with terminal cell differentiation because incubation of HL-60 cells with an agent or condition that causes suppression of growth but not induction of differentiation does not result in expression of the PC. At concentrations of 5-15 nM, the purified PC inhibited the growth of HL-60 cells and THP-1 cells, as well as other cell types belonging to different cell lineages. This growth inhibition was preceded by a reduction in [32P]phosphate incorporation and, at the higher PC concentrations, was associated with a reduction in [3H]thymidine, [3H]uridine, and [32S]methionine incorporation. The specific expression pattern and growth-inhibitory character of the PC suggests that the complex may have a role in suppressing cell growth during monomyelocytic terminal differentiation induced by specific chemical stimuli and during physiological and pathological events associated with monomyelocytic cell functions.
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pubmed:language |
eng
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Calgranulin A,
http://linkedlifedata.com/resource/pubmed/chemical/Calgranulin B,
http://linkedlifedata.com/resource/pubmed/chemical/Casein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Methionine,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/Uridine
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pubmed:status |
MEDLINE
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pubmed:author |
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
447-54
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2278876-Calcium-Binding Proteins,
pubmed-meshheading:2278876-Calgranulin A,
pubmed-meshheading:2278876-Calgranulin B,
pubmed-meshheading:2278876-Casein Kinases,
pubmed-meshheading:2278876-Cell Differentiation,
pubmed-meshheading:2278876-DNA, Neoplasm,
pubmed-meshheading:2278876-Growth Inhibitors,
pubmed-meshheading:2278876-Humans,
pubmed-meshheading:2278876-Leukemia, Myeloid,
pubmed-meshheading:2278876-Leukemia, Promyelocytic, Acute,
pubmed-meshheading:2278876-Methionine,
pubmed-meshheading:2278876-Neoplasm Proteins,
pubmed-meshheading:2278876-Phosphates,
pubmed-meshheading:2278876-Protein Kinase Inhibitors,
pubmed-meshheading:2278876-RNA, Neoplasm,
pubmed-meshheading:2278876-Ribonucleotides,
pubmed-meshheading:2278876-Thymidine,
pubmed-meshheading:2278876-Tumor Cells, Cultured,
pubmed-meshheading:2278876-Uridine
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pubmed:articleTitle |
A protein complex expressed during terminal differentiation of monomyelocytic cells is an inhibitor of cell growth.
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pubmed:affiliation |
Biological and Medical Research Division, Argonne National Laboratory, Illinois 60439.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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