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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1991-3-5
|
| pubmed:abstractText |
Normal phase high-performance liquid chromatography has been achieved of the common activated forms of valine on a LiChrosorb-CN or a muPorasil (underivatized silica) column using hexane containing 1.5 or 5% tert.-butanol as solvent. Compounds examined include the 2-alkoxy-5(4H)-oxazolones and symmetrical and mixed anhydrides of N-tert.-butoxycarbonyl-, N-benzyloxycarbonyl-, and N-9-fluorenylmethoxycarbonylvaline, the chloride of the latter, a p-nitrophenyl ester, 2-methyl-4-isopropyl-5(4H)-oxazolone, valine-N-carboxyanhydride, and the N- and O-ethoxycarbonyl adducts of l-hydroxybenzotriazole. The 5(4H)-oxazolones from N-tert.-butoxycarbonylvaline and N-benzyloxycarbonylglycylvaline decomposed during chromatography on the muPorasil but not the LiChrosorb-CN column. The method allows direct monitoring of reactions involving generation or consumption of activated forms of amino acids.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0367-8377
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
36
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
476-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1990
|
| pubmed:articleTitle |
High-performance liquid chromatography of 2,4-disubstituted-5(4H)-oxazolones, anhydrides and other activated forms of N-acyl- and N-alkoxycarbonylamino acids.
|
| pubmed:affiliation |
Department of Biochemistry, University of Ottawa, Ontario, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|