Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1991-2-26
|
pubmed:abstractText |
Recently flow cytometry techniques have been developed to replace the microscope reticulocyte count. We used thiazole orange, a RNA binding fluorochrome, to discriminate reticulocytes from mature erythrocytes. Thiazole orange and the Retic-COUNT software package were evaluated for performance of routine analysis on different flow instruments. The applied methodology analysed 10(4) cells semi-automatically in an easily performed manner. Consistent results were obtained with dipotassium EDTA anticoagulated blood (stable for 30 h after venesection), with incubation times in thiazole orange solution ranging from 2 to 7 h at 25 degrees C. This allowed flexibility in specimen collection and storage and assay performance with no change in results. Changes of incubation temperature up to 30 degrees C had no measurable effect. The values obtained showed good linearity, precision and accuracy for normal, low and high reticulocyte counts. However interferences were observed: RBC autofluorescence, nucleated RBC, Howell-Jolly bodies, high leucocyte count, high platelet count and giant platelets, all falsely increased the number of reticulocytes. These artifacts were eliminated by software gate corrections, thus leaving less than 5% of the specimen to be reanalysed by the microscopic method. The thiazole orange flow cytometric method was determined to be a fast, reliable method for the routine clinical quantitation of reticulocytes.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzothiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Quinolines,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/thiazole orange
|
pubmed:status |
MEDLINE
|
pubmed:issn |
0141-9854
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
12
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
287-99
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:2272158-Benzothiazoles,
pubmed-meshheading:2272158-Erythrocyte Count,
pubmed-meshheading:2272158-Flow Cytometry,
pubmed-meshheading:2272158-Fluorescent Dyes,
pubmed-meshheading:2272158-Humans,
pubmed-meshheading:2272158-Microscopy,
pubmed-meshheading:2272158-Quinolines,
pubmed-meshheading:2272158-Reproducibility of Results,
pubmed-meshheading:2272158-Reticulocytes,
pubmed-meshheading:2272158-Sensitivity and Specificity,
pubmed-meshheading:2272158-Software,
pubmed-meshheading:2272158-Thiazoles
|
pubmed:year |
1990
|
pubmed:articleTitle |
Reticulocyte count using thiazole orange. A flow cytometry method.
|
pubmed:affiliation |
University Hospital Leuven, Department of Haematology, Belgium.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|