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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
19
|
pubmed:dateCreated |
1979-12-18
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pubmed:abstractText |
The ATP required for in vitro transcription by vesicular stomatitis virus can be replaced by the analogue adenosine-5'-O-(3-thiotriphosphate) without affecting the rate of transcription or the nature of the mRNA products. RNA chains (less than 5 S) initiated with adenosine-5'-O-(3-thiotriphosphate) can be isolated by affinity chromatography on mercury-agarose columns used in these experiments. At least 80% of the leader RNA synthesized in vitro by infectious B virions and 70% of the leader RNA synthesized by defective-interfering particles of vesicular stomatitis virus contained a 5'-terminal triphosphate as judged by binding to mercury-agarose of leader RNA synthesized in the presence of the 5'-thiotriphosphate adenosine analogue.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
|
pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
10
|
pubmed:volume |
254
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
9339-41
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:226514-Adenosine Triphosphate,
pubmed-meshheading:226514-Affinity Labels,
pubmed-meshheading:226514-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:226514-Kinetics,
pubmed-meshheading:226514-Molecular Weight,
pubmed-meshheading:226514-RNA, Viral,
pubmed-meshheading:226514-Thionucleotides,
pubmed-meshheading:226514-Vesicular stomatitis Indiana virus
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pubmed:year |
1979
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pubmed:articleTitle |
Adenosine-5'-O-(3-thiotriphosphate) as an affinity probe for studying leader RNA's transcribed by vesicular stomatitis virus.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|