| pubmed-article:2264931 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2264931 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:2264931 | lifeskim:mentions | umls-concept:C0023884 | lld:lifeskim |
| pubmed-article:2264931 | lifeskim:mentions | umls-concept:C0205177 | lld:lifeskim |
| pubmed-article:2264931 | lifeskim:mentions | umls-concept:C1521827 | lld:lifeskim |
| pubmed-article:2264931 | lifeskim:mentions | umls-concept:C1518434 | lld:lifeskim |
| pubmed-article:2264931 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:2264931 | pubmed:dateCreated | 1991-2-13 | lld:pubmed |
| pubmed-article:2264931 | pubmed:abstractText | We have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts. | lld:pubmed |
| pubmed-article:2264931 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2264931 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2264931 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2264931 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2264931 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2264931 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2264931 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:2264931 | pubmed:issn | 1044-5498 | lld:pubmed |
| pubmed-article:2264931 | pubmed:author | pubmed-author:HattoriMM | lld:pubmed |
| pubmed-article:2264931 | pubmed:author | pubmed-author:BrennerD ADA | lld:pubmed |
| pubmed-article:2264931 | pubmed:author | pubmed-author:KarinMM | lld:pubmed |
| pubmed-article:2264931 | pubmed:author | pubmed-author:TugoresAA | lld:pubmed |
| pubmed-article:2264931 | pubmed:author | pubmed-author:VelozLL | lld:pubmed |
| pubmed-article:2264931 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2264931 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:2264931 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2264931 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2264931 | pubmed:pagination | 777-81 | lld:pubmed |
| pubmed-article:2264931 | pubmed:dateRevised | 2006-5-1 | lld:pubmed |
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| pubmed-article:2264931 | pubmed:meshHeading | pubmed-meshheading:2264931-... | lld:pubmed |
| pubmed-article:2264931 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2264931 | pubmed:articleTitle | A simplified method for the preparation of transcriptionally active liver nuclear extracts. | lld:pubmed |
| pubmed-article:2264931 | pubmed:affiliation | Department of Medicine, University of California, San Diego, La Jolla 92093. | lld:pubmed |
| pubmed-article:2264931 | pubmed:publicationType | Journal Article | lld:pubmed |
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