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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1991-2-13
|
| pubmed:abstractText |
We have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
1044-5498
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
777-81
|
| pubmed:dateRevised |
2006-5-1
|
| pubmed:meshHeading |
pubmed-meshheading:2264931-Animals,
pubmed-meshheading:2264931-Base Sequence,
pubmed-meshheading:2264931-Cell Nucleus,
pubmed-meshheading:2264931-Cell-Free System,
pubmed-meshheading:2264931-DNA,
pubmed-meshheading:2264931-DNA-Binding Proteins,
pubmed-meshheading:2264931-Gene Expression Regulation,
pubmed-meshheading:2264931-Liver,
pubmed-meshheading:2264931-Molecular Sequence Data,
pubmed-meshheading:2264931-Plasmids,
pubmed-meshheading:2264931-Rats,
pubmed-meshheading:2264931-Templates, Genetic,
pubmed-meshheading:2264931-Transcription, Genetic
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
A simplified method for the preparation of transcriptionally active liver nuclear extracts.
|
| pubmed:affiliation |
Department of Medicine, University of California, San Diego, La Jolla 92093.
|
| pubmed:publicationType |
Journal Article
|