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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1991-2-7
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pubmed:abstractText |
Intracellular stores of Ca2+ were mobilized transiently by carbachol in suspensions of electrically permeabilized SH-SY5Y cells. The kinetics and the dose-dependence of this mobilization paralleled carbachol-induced increases in inositol 1,4,5-trisphosphate (InsP3) mass [for both parameters EC50 (concn. giving half-maximal response) = 60-70 microM]. Guanosine 5'-[gamma-thio]triphosphate enhanced the maximal effect and the potency of carbachol on Ca2+ mobilization and InsP3 mass, but caused separation of the dose-response curves (EC50 = 0.6 microM and 5.6 microM respectively). These data show that functional coupling of muscarinic receptors to Ca2+ mobilization can be maintained after permeabilization, reveal major effects of guanine nucleotides on agonist-induced Ca2+ mobilization and provide a basis for explanation of discrepancies between agonist potency on InsP3 concentration and Ca2+ mobilization in intact cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-2153757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-2160274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-2302186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-2414285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-2537457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-2546303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-2550825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-2767136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-2818578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-2838362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-2904813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-2914616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-3062118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-3113327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-3304132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-3361307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-3491074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-6325442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-6412564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2264832-6607924
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
272
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
269-72
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pubmed:dateRevised |
2010-8-25
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pubmed:meshHeading |
pubmed-meshheading:2264832-Calcium,
pubmed-meshheading:2264832-Calcium Radioisotopes,
pubmed-meshheading:2264832-Carbachol,
pubmed-meshheading:2264832-Cell Line,
pubmed-meshheading:2264832-Cell Membrane Permeability,
pubmed-meshheading:2264832-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:2264832-Inositol Phosphates,
pubmed-meshheading:2264832-Kinetics,
pubmed-meshheading:2264832-Neuroblastoma,
pubmed-meshheading:2264832-Receptors, Muscarinic
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pubmed:year |
1990
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pubmed:articleTitle |
Coupling of muscarinic receptors to the mobilization of intracellular Ca2+ stores in permeabilized SH-SY5Y human neuroblastoma cells.
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pubmed:affiliation |
Department of Pharmacology and Therapeutics, University of Leicester, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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