Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
1991-2-7
pubmed:abstractText
We have demonstrated that mouse LTA cells synthesize cell-surface heparan sulfate proteoglycans (HSPGs) with regions of defined monosaccharide sequence that specifically interact with antithrombin (HSPGact). It remains unclear how HSPGact can be generated by a biosynthetic pathway with no simple template for directing the ordered assembly of monosaccharide units. To examine this issue, we treated LTA cells with ethyl methanesulfonate and then isolated seven stable mutants that synthesize only 8-27% of the wild-type HSPGact but produce normal amounts of other HSPGs. These mutants are recessive in nature and fall into at least two different complementation groups. The delineation of the molecular basis of these defects should help to elucidate the manner by which cells synthesize HSPGs with regions of defined monosaccharide sequence.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-1097111, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-1267803, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-14056989, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-2524478, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-2525126, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-277908, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-286307, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-2940242, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-2951371, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-2987678, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-3156864, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-3427019, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-3523146, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-3782085, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-3858816, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-3965453, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-3970699, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-4084555, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-4243761, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-4738234, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-6306651, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-6525337, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-6651824, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-6746897, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-6885772, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-6935668, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-6940150, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-6954474, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-6961402, http://linkedlifedata.com/resource/pubmed/commentcorrection/2263629-7053378
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9784-8
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Cell mutants defective in synthesizing a heparan sulfate proteoglycan with regions of defined monosaccharide sequence.
pubmed:affiliation
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't