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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1991-2-7
|
| pubmed:abstractText |
bcl-2 is a marker for the translocation t(14;18)(q32;q21) indicative of follicular B-cell lymphoma. We studied 115 cases of lymphoproliferative disease with the polymerase chain reaction for bcl-2 oncogene using biotin and radiolabeled probes to the major breakpoint and minor cluster regions. Twenty-three percent of B-cell lymphomas were positive for bcl-2. These included 12 of 20 cases of nodular follicular center cell lymphoma (nine small cleaved cell, one mixed small and large cell, and two large cell types). bcl-2 translocation was detected in only three of 45 cases of diffuse B-cell lymphoma, and cases of AIDS-related malignant lymphoma, monocytoid B-cell lymphoma, and mantle zone lymphoma were all negative. Nonneoplastic lymphoid proliferations were negative for bcl-2 including nine cases of abnormal follicular hyperplasia from patients with acquired immunodeficiency syndrome (AIDS) and AIDS-related complex. Cases of T-cell lymphoma and five cases of Hodgkin's disease were also negative. The polymerase chain reaction for bcl-2 is a rapid, sensitive technique in the evaluation of follicular B-cell proliferations, and the use of biotinylated probes and the alkaline phosphatase reaction eliminates the requirement for radioactive reagents.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0893-3952
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
3
|
| pubmed:geneSymbol |
bcl-2
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
659-63
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2263590-AIDS-Related Complex,
pubmed-meshheading:2263590-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:2263590-Base Sequence,
pubmed-meshheading:2263590-Blotting, Southern,
pubmed-meshheading:2263590-Chromosomes, Human, Pair 14,
pubmed-meshheading:2263590-Chromosomes, Human, Pair 18,
pubmed-meshheading:2263590-DNA, Neoplasm,
pubmed-meshheading:2263590-Hodgkin Disease,
pubmed-meshheading:2263590-Humans,
pubmed-meshheading:2263590-Hyperplasia,
pubmed-meshheading:2263590-Lymph Nodes,
pubmed-meshheading:2263590-Lymphoma,
pubmed-meshheading:2263590-Lymphoma, B-Cell,
pubmed-meshheading:2263590-Lymphoma, T-Cell,
pubmed-meshheading:2263590-Molecular Sequence Data,
pubmed-meshheading:2263590-Polymerase Chain Reaction,
pubmed-meshheading:2263590-Proto-Oncogene Proteins,
pubmed-meshheading:2263590-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:2263590-Translocation, Genetic
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Polymerase chain reaction for bcl-2 in diagnostic lymph node biopsies.
|
| pubmed:affiliation |
Department of Pathology and Laboratory Medicine, Cedars Sinai Medical Center, Los Angeles, California.
|
| pubmed:publicationType |
Journal Article
|