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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1979-11-21
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pubmed:abstractText |
A technique for selecting herpes simplex viruses expressing the viral thymidine kinase (TK+) from a population of predominantly TK- viruses was developed. This was accomplished by infecting TK- cells and incubating the cultures under a liquid overlay medium containing methotrexate. Since the TK- cells survive in this medium for only a limited period of time, it was necessary to add fresh uninfected TK- cells 48 h after infection. The technique allowed the detection and quantitation of the TK+ virus fraction in mixtures of TK+ and TK- viruses where the TK+ fraction was present in frequencies as low as 10(-5). It was also used to estimate reversion frequencies and to obtain and analyze TK+ revertants from TK- mutant strains of herpes simplex virus type 1.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-13744520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-14155835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-14166109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-165148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-169027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-172894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-193108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-194704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-194705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-198581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-199739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-205996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-207433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-208776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-210957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-212602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-4300104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-4327589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-4359421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-4361460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-4372299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/225554-940549
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
281-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1979
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pubmed:articleTitle |
Selective assay for herpes simplex viruses expressing thymidine kinase.
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pubmed:publicationType |
Journal Article
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