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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2-3
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pubmed:dateCreated |
1991-1-22
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pubmed:abstractText |
The highly selective 5-HT3 receptor antagonist [3H]GR65630 has been used to characterize 5-HT3 receptors in intact N1E-115 neuroblastoma cells. Equilibrium binding analysis demonstrated high-affinity binding to a single class of receptors with a Kd of 0.69 (+/- 0.12) nM and Bmax of 31.4 (+/- 11.4) fmol/10(5) cells, equivalent to approximately 200,000 sites per cell. Specific binding was displaced by low concentrations of 5-HT3-selective ligands, and by the nicotinic antagonist d-tubocurarine.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0014-2999
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
18
|
|
pubmed:volume |
189
|
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
223-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2253704-Animals,
pubmed-meshheading:2253704-Binding, Competitive,
pubmed-meshheading:2253704-Imidazoles,
pubmed-meshheading:2253704-Indoles,
pubmed-meshheading:2253704-Kinetics,
pubmed-meshheading:2253704-Ligands,
pubmed-meshheading:2253704-Mice,
pubmed-meshheading:2253704-Neuroblastoma,
pubmed-meshheading:2253704-Receptors, Serotonin,
pubmed-meshheading:2253704-Serotonin Antagonists,
pubmed-meshheading:2253704-Tumor Cells, Cultured
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pubmed:year |
1990
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pubmed:articleTitle |
Characterization of 5-HT3 receptors in intact N1E-115 neuroblastoma cells.
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pubmed:affiliation |
Molecular Neurobiology Unit, MRC Centre, Cambridge, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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