Linked Life Data

2251116

Source:http://linkedlifedata.com/resource/pubmed/id/2251116

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1991-1-15
pubmed:abstractText
An RNA synthesized in vitro was positioned on the Escherichia coli ribosome at the P site with tRNAala, and with a termination codon, UAA, as the next codon in the A site. Such a complex bound stoichiometric amounts of release factor 2 (RF-2); a corresponding RNA with UAC in place of UAA was not a template for the factor. An RNA containing 4-thio-UAA in place of the UAA supported binding of RF-2, and this has allowed site-directed crosslinking from the first position of the termination codon to answer two long standing questions about the termination of protein biosynthesis, the position of the termination codon and its proximity to the release factor during codon recognition. An RF-2.mRNA crosslinked product was detected, indicating the release factor and the termination codon are in close physical contact during the codon recognition event of termination. The 4-thio-U crosslinked also to the ribosome but only to the 30S subunit, and the proteins and the rRNA site concerned were identified. RF-2 decreased significantly the crosslinking to the ribosomal components, but no new crosslink sites were found. If the stop codon was deliberately displaced from the decoding site by one codon's length then a different pattern of crosslinking in particular to the rRNA resulted. These observations are consistent with a model of codon recognition by RF-2 at the decoding site, without a major shift in position of the codon.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2251116-2181146, http://linkedlifedata.com/resource/pubmed/commentcorrection/2251116-2464693, http://linkedlifedata.com/resource/pubmed/commentcorrection/2251116-2651438, http://linkedlifedata.com/resource/pubmed/commentcorrection/2251116-2690012, http://linkedlifedata.com/resource/pubmed/commentcorrection/2251116-2714270, http://linkedlifedata.com/resource/pubmed/commentcorrection/2251116-2953976, http://linkedlifedata.com/resource/pubmed/commentcorrection/2251116-3048381, http://linkedlifedata.com/resource/pubmed/commentcorrection/2251116-3283702, http://linkedlifedata.com/resource/pubmed/commentcorrection/2251116-3288986, http://linkedlifedata.com/resource/pubmed/commentcorrection/2251116-3297159, http://linkedlifedata.com/resource/pubmed/commentcorrection/2251116-4598299, http://linkedlifedata.com/resource/pubmed/commentcorrection/2251116-6350288, http://linkedlifedata.com/resource/pubmed/commentcorrection/2251116-6381487, http://linkedlifedata.com/resource/pubmed/commentcorrection/2251116-6802827
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6537-44
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Codon recognition in polypeptide chain termination: site directed crosslinking of termination codon to Escherichia coli release factor 2.
pubmed:affiliation
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't