Linked Life Data

2247336

Source:http://linkedlifedata.com/resource/pubmed/id/2247336

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1991-1-10
pubmed:abstractText
Regulation of intracellular free calcium ([Ca2+]i) in single epithelial duct cells of isolated rat and guinea pig pancreatic interlobular ducts by secretin, carbachol and cholecystokinin was studied by microspectrofluorometry using the Ca2(+)-sensitive, fluorescent probe Fura-2. Rat and guinea pig duct cells exhibited mean resting [Ca2+]i of 84 nM and 61 nM, respectively, which increased by 50%-100% in response to carbachol stimulation, thus demonstrating the presence of physiologically responsive cholinergic receptors in pancreatic ducts of both species. The carbachol-induced increase in [Ca2+]i involved both mobilization of Ca2+ from intracellular stores and stimulation of influx of extracellular Ca2+. In contrast, neither cholecystokinin nor secretin showed reproducible or sizeable increases in [Ca2+]i. Both rat and guinea pig duct cells showed considerable resting Ca2+ permeability. Lowering or raising the extracellular [Ca2+]i led, respectively, to a decrease or increase in the resting [Ca2+]i. Application of Mn2+ resulted in a quenching of the fluorescence signal indicating its entry into the cell. The resting Ca2+ and Mn2+ permeability could be blocked by La3+ suggesting that it is mediated by a Ca2+ channel.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
416
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
652-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Secretagogue effects on intracellular calcium in pancreatic duct cells.
pubmed:affiliation
Department of Physiology, University of Michigan, Ann Arbor 48109-0622.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.