Linked Life Data

2245650

Source:http://linkedlifedata.com/resource/pubmed/id/2245650

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1991-1-4
pubmed:abstractText
To better define the growth requirements for corneal epithelial cells, methods for serum-free culture were established. Sheets of corneal epithelium from eyes of swine and rabbits were obtained by dispase treatment of corneal buttons, and single cells and small clumps were obtained by further dissociation with trypsin/EDTA. Growth of cells with epithelial-like morphology was readily achieved in low calcium MCDB 153 medium containing epidermal growth factor, insulin, hydrocortisone, and bovine pituitary extract. Primary cultures could be subcultivated at least four times at 1:6 split ratios. Examination of cultured corneal epithelial cells by transmission electron microscopy demonstrated a relatively undifferentiated phenotype. Elevation of calcium in the medium caused the reappearance of desmosomal junctions and a more typical corneal epithelial morphology.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0271-3683
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
919-23
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Serum-free culture of porcine and rabbit corneal epithelial cells.
pubmed:affiliation
School of Optometry, University of California, Berkeley.
pubmed:publicationType
Journal Article