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rdf:type |
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lifeskim:mentions |
umls-concept:C0008300,
umls-concept:C0030016,
umls-concept:C0031617,
umls-concept:C0034865,
umls-concept:C0106006,
umls-concept:C0181496,
umls-concept:C0182400,
umls-concept:C0230840,
umls-concept:C1373200,
umls-concept:C1522485,
umls-concept:C1546465,
umls-concept:C1622418,
umls-concept:C1705175,
umls-concept:C1705176,
umls-concept:C1705177,
umls-concept:C1705178,
umls-concept:C1705425,
umls-concept:C1882348,
umls-concept:C1882726,
umls-concept:C2603343
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|
pubmed:issue |
3
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|
pubmed:dateCreated |
1979-10-17
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|
pubmed:abstractText |
We find from studying the inhibitory effect of N-ethylmaleimide (NEM) on the enzymatic activity of beta-hydroxybutyrate dehydrogenase, that approximately one molecule of NEM is bound for one molecule of protein when the enzymatic activity is completely inhibited. Since the protein is a dimer this implies that each molecule of protein possesses only one thiol group in its catalytic center. Two long chain maleimide derivates: (10.3) NEM and (1.14) NEM conform, if a reasonable assumption is accepted to the conditions required for the study of the recombination of beta-hydroxybutyrate dehydrogenase with lecithin vesicles by spin label technique.
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pubmed:language |
fre
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0567-655X
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
288
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
363-5
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:223776-Animals,
pubmed-meshheading:223776-Binding Sites,
pubmed-meshheading:223776-Electron Spin Resonance Spectroscopy,
pubmed-meshheading:223776-Ethylmaleimide,
pubmed-meshheading:223776-Hydroxybutyrate Dehydrogenase,
pubmed-meshheading:223776-Iodoacetamide,
pubmed-meshheading:223776-Maleimides,
pubmed-meshheading:223776-Mitochondria, Liver,
pubmed-meshheading:223776-Phosphatidylcholines,
pubmed-meshheading:223776-Rats,
pubmed-meshheading:223776-Spin Labels
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pubmed:year |
1979
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pubmed:articleTitle |
[Choice of a spin label probe in order to study the recombination of beta-hydroxybutyrate dehydrogenase of a rat liver inner mitochondrial membrane with lecithin vesicles].
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pubmed:publicationType |
Journal Article,
English Abstract
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