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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1990-12-4
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pubmed:abstractText |
The protooncogene c-myb encodes a nuclear transcription factor that binds to DNA in a sequence-specific manner and transactivates transcription of several viral and cellular genes. The expression of c-myb is induced in mitogen-stimulated peripheral blood lymphocytes and is constitutively expressed in several CD4+ T-cell and myeloid cell lines, all of which constitute excellent targets for human immunodeficiency virus (HIV) infection and replication. We looked for the presence of Myb-binding motifs in human retroviral long terminal repeats (LTRs) and tested for Myb binding to HIV-1 LTR sequences by using a highly purified recombinant Myb protein. Our results show that HIV-1 LTR contains one high-affinity Myb-binding site along with two or more low-affinity binding sites. DNase I protection analysis as well as oligonucleotide competition experiments indicate that this binding is sequence specific. Introduction of purified Myb protein directly into HeLa cells harboring HIV-1 LTR chloramphenicol acetyltransferase vectors indicates that Myb protein transactivates HIV-1 LTR-mediated transcription. Thus, Myb protein binding to HIV LTR sequences may constitute one of the signals that regulates HIV-1 transcription.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-2184033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-2205850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-2418502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-2521508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-2558687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-265521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-2668947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-2688896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-2760991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-2795714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-2795721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-2849510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-2990040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-2994217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-2995487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-3001934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-3008338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-3031512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-3094962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-3138113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-3185713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-3428273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-3522356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-3523754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-3773731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-6083454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-6096719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-6189183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-6200936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-6206563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-6286010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-6334304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236022-6960240
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8090-4
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2236022-Base Sequence,
pubmed-meshheading:2236022-DNA, Viral,
pubmed-meshheading:2236022-Genes, Viral,
pubmed-meshheading:2236022-HIV-1,
pubmed-meshheading:2236022-HIV-2,
pubmed-meshheading:2236022-HeLa Cells,
pubmed-meshheading:2236022-Human T-lymphotropic virus 1,
pubmed-meshheading:2236022-Human T-lymphotropic virus 2,
pubmed-meshheading:2236022-Molecular Sequence Data,
pubmed-meshheading:2236022-Oncogene Proteins v-myb,
pubmed-meshheading:2236022-Oncogenes,
pubmed-meshheading:2236022-Plasmids,
pubmed-meshheading:2236022-Protein Binding,
pubmed-meshheading:2236022-Protein-Tyrosine Kinases,
pubmed-meshheading:2236022-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:2236022-Retroviridae Proteins, Oncogenic,
pubmed-meshheading:2236022-Transcription, Genetic,
pubmed-meshheading:2236022-Transcriptional Activation
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pubmed:year |
1990
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pubmed:articleTitle |
Myb protein binds to human immunodeficiency virus 1 long terminal repeat (LTR) sequences and transactivates LTR-mediated transcription.
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pubmed:affiliation |
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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