Linked Life Data

2232866

Source:http://linkedlifedata.com/resource/pubmed/id/2232866

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1990-12-5
pubmed:abstractText
This paper describes a new medium which promotes myelin formation in cultures of rat dorsal root ganglia, and controls fibroblast proliferation. The medium, medium 'M', contains D-valine rather than L-valine and is added to cultures after seven days in vitro (DIV). The presence of D-amino acid oxidase in Schwann cells and neurons permits these cells to convert D-valine to L-valine, an essential amino-acid. Fibroblasts however lack D-amino acid oxidase but as some L-valine is present in the fetal calf serum component of medium 'M' those fibroblasts already present in the cultures by day 7 are maintained. Further fibroblast proliferation is however inhibited. The use of medium 'M' does not require the use of specific antimitotic agents making it cheaper and quicker to obtain myelinated cultures than with previous methods. The medium results in widespread myelin formation by 21 DIV. Cultures may be grown on plastic or collagen coated glass coverslips.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-55
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
A new medium for in vitro peripheral nervous tissue myelination without the use of antimitotics.
pubmed:affiliation
School of Biological Sciences, University of Sydney, NSW, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't