Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-12-26
pubmed:abstractText
Interleukin-1 (IL-1) plays a major role in inflammatory responses. Activation of coagulation and fibrin deposition typical of these reactions is mediated by macrophage procoagulants induced on stimulated macrophages. IL-1 activity in the supernatant of lipopolysaccharide (LPS)-stimulated guinea-pig macrophages was markedly enhanced by the presence of thrombin during macrophage activation. Although thrombin alone had no effect, inclusion of 1 mU/ml of thrombin with suboptimal levels of LPS produced a 200-fold increase in IL-1 activity, and further enhancement was observed with increasing doses of thrombin. The active site of thrombin was necessary for enhancement, as the serine esterase inhibitor di-isopropyl-fluorophosphate (DIP) and hirudin inhibited the synergy observed with LPS and thrombin. Prothrombin and Factor Xa also enhanced IL-1 production, although not to the same extent as thrombin. Factor Xa-like activity was demonstrated on the surface of LPS-stimulated macrophages. Both the Xa-like activity and IL-1 generated by LPS-stimulated cells were inhibited by heparin. Heparin with a high affinity for antithrombin III (anti-coagulant heparin; HAH) inhibited IL-1 generation, whereas low-affinity heparin (non-anticoagulant; LAH) had no effect. We show that proteases of the extrinsic coagulation cascade enhance IL-1 generation and propose that a Factor Xa-like activity present in activated macrophages, together with thrombin, may be important in IL-1 processing.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-13938029, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-2420874, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-2457619, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-2783440, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-2910358, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-3072553, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-3100431, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-3277884, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-3301639, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-3345343, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-3491144, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-3492551, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-3495602, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-3496917, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-3543052, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-3879529, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-3919388, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6020011, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6049105, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6083565, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6167623, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6167624, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6208212, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6209582, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6325057, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6352100, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6368733, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6395409, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6398242, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6398244, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6413584, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6757715, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6780198, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6863244, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6971397, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-6980899, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-7150243, http://linkedlifedata.com/resource/pubmed/commentcorrection/2228024-7200121
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0019-2805
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
236-41
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Thrombin and factor Xa enhance the production of interleukin-1.
pubmed:affiliation
Kolling Institute of Medical Research, Royal North Shore Hospital, St. Leonards, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't