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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1979-9-17
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pubmed:abstractText |
A corticotropin antiserum was obtained from rabbits immunized with synthetic 1--24 corticotropin conjugated with bovine serum albumin. The antiserum did not cross react with synthetic alpha-melanotropin or with synthetic beta-endorphin and had a cross reactivity of 0.23% with human beta-lipotropin. We developed a radioimmunoassay with the antiserum obtained, in which we used polyethylene glycol in conjunction with a second precipitating antibody for fast (15-min) separation of antibody-bound and free corticotropin. The assay had a sensitivity of 16 ng/L and was validated on patients with various pituitary and adrenal diseases. From 103 normal subjects, the median value for corticotropin in specimens collected during the morning was 34 ng/L of plasma; the upper 95% confidence limit of the normal range was 98 ng/L.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0009-9147
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1267-73
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:222503-Adrenal Gland Diseases,
pubmed-meshheading:222503-Adrenocorticotropic Hormone,
pubmed-meshheading:222503-Animals,
pubmed-meshheading:222503-Cosyntropin,
pubmed-meshheading:222503-Cross Reactions,
pubmed-meshheading:222503-Female,
pubmed-meshheading:222503-Humans,
pubmed-meshheading:222503-Hypoglycemia,
pubmed-meshheading:222503-Immune Sera,
pubmed-meshheading:222503-Insulin,
pubmed-meshheading:222503-Iodine Radioisotopes,
pubmed-meshheading:222503-Male,
pubmed-meshheading:222503-Neoplasms,
pubmed-meshheading:222503-Rabbits,
pubmed-meshheading:222503-Radioimmunoassay,
pubmed-meshheading:222503-Reference Values
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pubmed:year |
1979
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pubmed:articleTitle |
Human corticotropin (ACTH) radioimmunoassay with synthetic 1--24 ACTH.
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pubmed:publicationType |
Journal Article
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