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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1979-9-25
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J02288,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J02290,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J02291,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/J02292,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/K00932,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/K00997,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/K01041,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/K01071,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/K01072,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/V01117,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/V01147
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pubmed:abstractText |
We have investigated the DNA sequence alterations in several hr-t mutants of polyoma virus. These mutants are defective in one of the two known viral functions essential for transformation and are altered with respect to several minor T antigen species. The lesions in some of these mutants have been mapped previously by marker rescue experiments to Hpa II fragment 4 (Hpa II-4, 78.4--91.7 map units) in the proximal part of the early region of the viral DNA. Thirteen of sixteen hr-t mutants examined carry deletions 2 to 5 map units (100--250 bp) long in Hpa 11-4. Three mutants carry either point mutations or very small deletions/insertions. Eight of the deletion mutants were mapped closely with restriction enzymes. Seven of them have deletions located entirely within the Hae III subfragment A of Hpa II-4 (the Hae A subfragment, 78.4--85.2 map units), and one extends just beyond this subfragment, ending at 85.5 map units. The complete sequence of the wild-type Hae A subfragment was determined and compared with those of four deletion mutants, NG-18, A-8, 6B5 and B-2. The deletion in each of these mutants is out-of-phase: NG-18, 187 bp; A-8, 127 bp; 6B-5, 179 bp; B-2, 241 bp. All are expected to remove protein sequences in the C terminal part of the small t antigen.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
|
pubmed:issn |
0092-8674
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
16
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
505-13
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:222464-Amino Acid Sequence,
pubmed-meshheading:222464-Antigens, Neoplasm,
pubmed-meshheading:222464-Base Sequence,
pubmed-meshheading:222464-Cell Transformation, Viral,
pubmed-meshheading:222464-Chromosome Deletion,
pubmed-meshheading:222464-DNA, Viral,
pubmed-meshheading:222464-DNA Restriction Enzymes,
pubmed-meshheading:222464-Genes,
pubmed-meshheading:222464-Genes, Viral,
pubmed-meshheading:222464-Mutation,
pubmed-meshheading:222464-Polyomavirus,
pubmed-meshheading:222464-Viral Proteins
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pubmed:year |
1979
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pubmed:articleTitle |
DNA sequence alterations in Hr-t deletion mutants of polyoma virus.
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pubmed:publicationType |
Journal Article
|