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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1990-11-14
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pubmed:abstractText |
Sparse (75 to 2000 cells/cm2) density cultures of normal human bronchial epithelial cells uniformly undergo terminal squamous differentiation when incubated in medium containing serum (fetal bovine serum [FBS]) or transforming growth factor beta-1 (TGF-beta 1). It was found that the cell density of the culture affects the probability that a cell will respond to these differentiation-inducing agents. Thus whereas irreversible inhibition of DNA synthesis occurs in sparse cell-density cultures within 24 hours after exposure, only a transient (less than 36 hours) depression in DNA synthesis was seen in high (more than 10,000 cells/cm2) density cultures. In addition, although phase microscopic image analysis revealed that virtually all of the cells displayed a squamous morphology within 1 hour after exposure to FBS or TGF-beta 1, observations made 48 to 72 hours later showed the presence of clusters of small prolate spheroid-shaped cells surrounded by many involucrin-positive squamous-appearing cells. Only the small cells were capable of DNA synthesis and cell division as determined by autoradiography and time-lapse photomicrographic images. These replicating cells immediately undergo squamous differentiation if they are subcultured and reinoculated at low cell density and incubated in medium supplemented with FBS or TGF-beta 1. Therefore the probability that a human bronchial epithelial cell will be refractive to FBS- or TGF-beta 1 induced terminal squamous differentiation is solely a function of the cell density of the culture.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-1009573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-2415144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-2417407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-2427381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-2436229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-2450641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-2461952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-2463113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-2464440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-2578881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-2645058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-2846394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-2871553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-2887577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-3022564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-3475277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-3491081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-3539318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-3652386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-3815521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-3975607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-6188787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-6191883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-6276417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-6360848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-6640540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-6698333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2221015-7141447
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0002-9440
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
137
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
833-43
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2221015-Autoradiography,
pubmed-meshheading:2221015-Bronchi,
pubmed-meshheading:2221015-Cell Count,
pubmed-meshheading:2221015-Cell Cycle,
pubmed-meshheading:2221015-Cell Differentiation,
pubmed-meshheading:2221015-Cell Line,
pubmed-meshheading:2221015-Colony-Forming Units Assay,
pubmed-meshheading:2221015-DNA,
pubmed-meshheading:2221015-Epithelial Cells,
pubmed-meshheading:2221015-Epithelium,
pubmed-meshheading:2221015-Fetal Blood,
pubmed-meshheading:2221015-Humans,
pubmed-meshheading:2221015-Protein Precursors,
pubmed-meshheading:2221015-Transforming Growth Factor beta
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pubmed:year |
1990
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pubmed:articleTitle |
Cell density governs the ability of human bronchial epithelial cells to recognize serum and transforming growth factor beta-1 as squamous differentiation-inducing agents.
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pubmed:affiliation |
Division of Cancer Etiology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
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pubmed:publicationType |
Journal Article
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