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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1979-8-29
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pubmed:abstractText |
8-Azidoguanosine 3',5'-cyclic monophosphate (8-N3cGMP) has been synthesized for use as a photoactive probe for the labeling of cGMP receptors. The ability of 8-N3cGMP to be bound at specific cGMP binding sites was demonstrated by its ability to activate cGMP-dependent protein kinase isolated from bovine lung (Ka = 1.1 x 10(-7) M) and to inhibit competitively the binding of [3H] cGMP to the enzyme [Kd (8 N3GMP)/Kd (cGMP) = 6]. Photolysis of 8-N3[32P]cGMP in the presence of a crude enzyme preparation resulted in the covalent attachment of analog to cGMP-dependent protein kinase. Half-maximal labeling occurred at 2.2 x 10(-7) M. The incorporation of the analog was completely inhibited by the addition of cGMP.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-13398422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-169887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-170261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-186666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-193862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-197091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-197093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-198632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-207709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-210691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-214781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-234451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-237908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-270679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-4279407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-4298072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-4307314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-4323628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-4345087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-4372603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-4900513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-5445796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-5832284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-8775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/221918-909454
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2213-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
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pubmed:year |
1979
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pubmed:articleTitle |
Synthesis and use of 8-azidoguanosine 3',5'-cyclic monophosphate as photoaffinity label for cyclic GMP-dependent protein kinase.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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