Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
1990-11-5
pubmed:databankReference
pubmed:abstractText
We have determined the nucleotide sequence of the CABP1 gene from Dictyostelium discoideum. Together with previous data on cDNA sequences, we establish that alternative splicing of transcripts derived from this gene is responsible for the production of the two CABP1 subunits. RNA blot analysis suggested that alternative splicing of the CABP1 transcripts occurs during growth and throughout development. In addition, we have compiled the intron sequences of Dictyostelium pre-mRNAs and observed that the GUAAGU hexanucleotide at the 5' splice site is highly conserved. The 5' splice site of CABP1 deviates from the consensus hexanucleotide in having a sequence of GUAAUA. To assess the role of the modified 5' splice on differential splicing, we have constructed an actin-CABP1 fusion gene and transformed it into Dictyostelium cells. Analysis by immunoprecipitation, with anti-CABP1 antibody and amplification of specific cDNAs by polymerase chain reaction show that the transcripts generated by the fusion gene are alternatively spliced. When the 5' splice site of the fusion gene is mutated to conform to the consensus sequence, the resulting transcripts are constitutively spliced. These observations suggest that changes in positions 5 and 6 of the donor splice site are involved in the alternative splicing of the CABP1 transcripts.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2152923, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2153977, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2519618, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2540036, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2555685, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2601718, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2602140, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2701943, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2734099, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2758463, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2762118, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2779573, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2813371, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2827119, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2843819, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2897942, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-2986840, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3015938, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3017190, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3031453, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3031475, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3035502, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3304142, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3323811, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3386632, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3398920, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3436528, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3513966, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3600646, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3622778, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3678838, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3779836, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3817296, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3893746, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-3935321, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-5530748, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-6091907, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-6204770, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-6254843, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-6296682, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-6330566, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-6828377, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-6847617, http://linkedlifedata.com/resource/pubmed/commentcorrection/2216719-7465423
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5457-63
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:2216719-Actins, pubmed-meshheading:2216719-Amino Acid Sequence, pubmed-meshheading:2216719-Base Sequence, pubmed-meshheading:2216719-Blotting, Northern, pubmed-meshheading:2216719-Carrier Proteins, pubmed-meshheading:2216719-Cloning, Molecular, pubmed-meshheading:2216719-Cyclic AMP Receptor Protein, pubmed-meshheading:2216719-Dictyostelium, pubmed-meshheading:2216719-Genes, Fungal, pubmed-meshheading:2216719-Introns, pubmed-meshheading:2216719-Molecular Sequence Data, pubmed-meshheading:2216719-Polymerase Chain Reaction, pubmed-meshheading:2216719-Precipitin Tests, pubmed-meshheading:2216719-RNA Precursors, pubmed-meshheading:2216719-RNA Splicing, pubmed-meshheading:2216719-Restriction Mapping, pubmed-meshheading:2216719-Transcription, Genetic, pubmed-meshheading:2216719-Transformation, Genetic
pubmed:year
1990
pubmed:articleTitle
The molecular basis for alternative splicing of the CABP1 transcripts in Dictyostelium discoideum.
pubmed:affiliation
Department of Biology, McGill University, Montreal, Quebec, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't