Linked Life Data

22069700

Source:http://linkedlifedata.com/resource/pubmed/id/22069700

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-11-9
pubmed:abstractText
The frequency and severity of bacteremic infections has increased over the last decade and bacterial endovascular infections (i.e., sepsis or endocarditis) are associated with high morbidity and mortality. Bacteria or secreted bacterial products modulate platelet function and, as a result, affect platelet accumulation at sites of vascular infection and inflammation. However, whether bacterial products regulate synthetic events in platelets is not known. In the present study, we determined if prolonged contact with staphylococcal ?-toxin signals platelets to synthesize B-cell lymphoma (Bcl-3), a protein that regulates clot retraction in murine and human platelets. We show that ?-toxin induced ?(IIb)?(3)-dependent aggregation (EC(50) 2.98 µg/mL ± 0.64 µg/mL) and, over time, significantly altered platelet morphology and stimulated de novo accumulation of Bcl-3 protein in platelets. Adherence to collagen or fibrinogen also increased the expression of Bcl-3 protein by platelets. ?-toxin altered Bcl-3 protein expression patterns in platelets adherent to collagen, but not fibrinogen. Pretreatment of platelets with inhibitors of protein synthesis or the mammalian Target of Rapamycin (mTOR) decreased Bcl-3 protein expression in ?-toxin stimulated platelets. In conclusion, Staphylococcusaureus-derived ?-toxin, a pore forming exotoxin, exerts immediate (i.e., aggregation) and prolonged (i.e., protein synthesis) responses in platelets, which may contribute to increased thrombotic events associated with gram-positive sepsis or endocarditis.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Feb
pubmed:issn
2072-6651
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
120-33
pubmed:dateRevised
2011-11-14
pubmed:year
2011
pubmed:articleTitle
Staphylococcus aureus ?-Toxin Triggers the Synthesis of B-Cell Lymphoma 3 by Human Platelets.
pubmed:affiliation
Department of Medicine III, Martin Luther University, Halle, Saale, Germany; Email: schubert-sebastian@gmx.net (S.S.); monika.otto@medizin.uni-halle.de (M.O.); hagen.behr@medizin.uni-halle.de (H.B.); harald.Loppnow@medizin.uni-halle.de (H.L.); axel.schlitt@medizin.uni-halle.de (A.S.); martin.russ@medizin.uni-halle.de (M.R.); karl.werdan@medizin.uni-halle.de (K.W.).
pubmed:publicationType
Journal Article