Linked Life Data

22046185

Source:http://linkedlifedata.com/resource/pubmed/id/22046185

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-11-2
pubmed:abstractText
Caries development in the presence of Streptococcus mutans is associated not only with the production of extracellular water-insoluble polymers but also is based on water-soluble polysaccharides. The aim of this study was the evaluation of a novel glucan-specific Lectin assay for monitoring water-soluble EPS produced by S. mutans during several growth periods in different media. S. mutans cultures were grown for 24?h, 48?h, and 144?h in medium deficient of sucrose (A) and medium supplemented with 5% sucrose (B). Microtiter well plates were coated with cell-free supernatants followed by the addition of labeled Concanavalin-A and enzyme substrate. The substrate reactions were kinetically detected at 405?nm. The validation of the assay was performed using carbohydrates dextran, xanthan, and sucrose as reference. This new Concanavalin-A-based assay showed the highest sensitivity for dextran and revealed that the glucan production of S. mutans reached its maximum at 144?h in medium B according to bacterial maturation.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:issn
1687-8736
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
2011
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
435830
pubmed:year
2011
pubmed:articleTitle
Dynamic Production of Soluble Extracellular Polysaccharides by Streptococcus mutans.
pubmed:affiliation
Department of Operative Dentistry and Periodontology, Center of Dentistry, Oral Medicine, and Maxillofacial Surgery, Eberhard-Karls University Tübingen, 72076 Tübingen, Germany.
pubmed:publicationType
Journal Article