Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1990-10-18
|
| pubmed:abstractText |
In order to investigate the role of insulin as a potential target autoantigen of cellular immunity in the prediabetic period, proliferative responses of T lymphocytes to human insulin were studied in nine islet-cell antibody (ICA) + first-degree relatives of patients with Type I diabetes (individuals at high risk for the development of Type I diabetes, or the 'prediabetic' group, which was never treated with insulin) and in 12 control individuals. Insulin autoantibodies were present in 6/9 (67%) of the prediabetic subjects and none of the controls. Peripheral blood lymphocytes were collected on Ficoll and incubated with human insulin, control antigens, or media alone for 5-6-day and 9-10-day incubation periods. Cells were pulsed with 3H-thymidine, harvested, and analysed in a scintillation counter. Results are expressed as stimulation index (SI = cpm with antigen/cpm without antigen), with a SI greater than or equal to 1.5 considered a positive response. Eight of nine (89%) prediabetic individuals responded positively to insulin after a 9-10-day incubation period, in contrast to four of 12 (33%) control subjects, P less than 0.05. The mean proliferative response to insulin after 9-10 days' incubation was 2.1 +/- 0.4 and 1.2 +/- 0.1, for the prediabetic and control groups, respectively. The proliferative response to insulin was not directly correlated with levels of insulin autoantibodies (r = -0.05, NS). These data suggest that most individuals at high risk for the development of Type I diabetes display a cellular immune response to insulin, and a subset of these individuals does not display a concomitant humoral immune response to insulin based on the presence or absence of insulin autoantibodies.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0896-8411
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
3
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
321-7
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2204345-Adolescent,
pubmed-meshheading:2204345-Adult,
pubmed-meshheading:2204345-Aged,
pubmed-meshheading:2204345-Animals,
pubmed-meshheading:2204345-Autoantibodies,
pubmed-meshheading:2204345-Autoantigens,
pubmed-meshheading:2204345-Child,
pubmed-meshheading:2204345-Diabetes Mellitus, Type 1,
pubmed-meshheading:2204345-Humans,
pubmed-meshheading:2204345-Insulin,
pubmed-meshheading:2204345-Insulin Antibodies,
pubmed-meshheading:2204345-Islets of Langerhans,
pubmed-meshheading:2204345-Lymphocyte Activation,
pubmed-meshheading:2204345-Middle Aged,
pubmed-meshheading:2204345-Prediabetic State,
pubmed-meshheading:2204345-Rats,
pubmed-meshheading:2204345-Rats, Inbred Strains,
pubmed-meshheading:2204345-Risk Factors,
pubmed-meshheading:2204345-T-Lymphocytes
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Cellular immunity to human insulin in individuals at high risk for the development of type I diabetes mellitus.
|
| pubmed:affiliation |
Children's Hospital, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02115.
|
| pubmed:publicationType |
Journal Article
|