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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1990-10-11
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pubmed:abstractText |
Recombinant human IL 1 beta inhibits glucose-induced insulin secretion from isolated pancreatic islets and from purified beta-cells obtained by fluorescence-activated cell sorting (FACS) of dispersed islet cells. Brief (1 h) exposure of isolated islets to IL 1 produces sustained inhibition of insulin secretion for at least 17 h after the IL 1 has been removed from the culture medium. An inhibitory effect of IL 1 on insulin secretion is not observed when islets are coincubated with an inhibitor of DNA transcription (actinomycin D). This finding indicates that the inhibitory effect of IL 1 on insulin secretion requires transcription of one or more genes during the first hour of exposure of islets to IL 1. The inhibitory effect of IL 1 on insulin secretion also requires mRNA translation, because three structurally distinct inhibitors of protein synthesis (cycloheximide, anisomycin, and puromycin) prevent IL 1-induced inhibition of insulin secretion when added to islets after the 1-h exposure to IL 1. Two-dimensional gel electrophoresis of islet proteins metabolically labeled with [35S]methionine demonstrates that IL 1 augments the expression of a 65-kD (pl approximately 6.5) protein by greater than 2.5-fold. These findings indicate that biochemical events occurring within 1 h of exposure of islets to IL 1 lead to an inhibition of insulin secretion that persists for at least 17 h after the removal of IL 1. One of the early biochemical effects of IL 1 on islets is gene transcription (0-1 h), which is followed by mRNA translation (after 1 h). Our results suggest that the inhibitory effect of IL 1 on insulin secretion is mediated by protein(s) whose synthesis is induced by IL 1.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-2137789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-2405856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-2440648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-2453340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-2507377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-2527507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-2536318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-2608062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-2656294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-2662694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-3046964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-3121415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-3297891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-3308437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-3311860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-3514344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-3530029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-3530842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-3891788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-3901813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-4691265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-5033417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-6366468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-646093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-6621379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203826-6805324
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
856-63
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:2203826-Animals,
pubmed-meshheading:2203826-Cycloheximide,
pubmed-meshheading:2203826-Dactinomycin,
pubmed-meshheading:2203826-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:2203826-Gene Expression,
pubmed-meshheading:2203826-Glucose,
pubmed-meshheading:2203826-Insulin,
pubmed-meshheading:2203826-Interleukin-1,
pubmed-meshheading:2203826-Islets of Langerhans,
pubmed-meshheading:2203826-Isoelectric Point,
pubmed-meshheading:2203826-Molecular Weight,
pubmed-meshheading:2203826-Protein Biosynthesis,
pubmed-meshheading:2203826-RNA, Messenger,
pubmed-meshheading:2203826-Rats,
pubmed-meshheading:2203826-Rats, Inbred Strains,
pubmed-meshheading:2203826-Secretory Rate,
pubmed-meshheading:2203826-Time Factors,
pubmed-meshheading:2203826-Transcription, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
Interleukin 1 inhibits insulin secretion from isolated rat pancreatic islets by a process that requires gene transcription and mRNA translation.
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pubmed:affiliation |
Department of Pathology, Washington University School of Medicine, Saint Louis, Missouri 63110.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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