Linked Life Data

2203754

Source:http://linkedlifedata.com/resource/pubmed/id/2203754

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1990-10-9
pubmed:databankReference
pubmed:abstractText
The hutC gene of Klebsiella aerogenes encodes a repressor that regulates expression of the histidine utilization (hut) operons. The DNA sequence of a region known to contain hutC was determined and shown to contain two long rightward-reading open reading frames (ORFs). One of these ORFs was identified as the 3' portion of the hutG gene. The other ORF was the hutC gene. The repressor predicted from the hutC sequence contained a helix-turn-helix motif strongly similar to that seen in other DNA-binding proteins, such as lac repressor and the catabolite gene activator protein. This motif was located in the N-terminal portion of the protein, and this portion of the protein seemed to be sufficient to allow repression of the hutUH operon but insufficient to allow interaction with the inducer. The presence of a promoterlike sequence and a ribosome-binding site immediately upstream of the hutC gene explained the earlier observation that hutC can be transcribed independently of the other hut operon genes. The predicted amino acid sequence of hut repressor strongly resembled that of the corresponding protein from Pseudomonas putida (S. L. Allison and A. T. Phillips, J. Bacteriol. 172:5470-5476, 1990). An unexpected, leftward-reading ORF extending from about the middle of hutC into the preceding (hutG) gene was also detected. The deduced amino acid sequence of this leftward ORF was quite distinct from that of an unexpected ORF of similar size found immediately downstream of the P. putida hutC gene. The nonstandard codon usage of this leftward ORF and the expression of repressor activity from plasmids with deletions in this region made it unlikely that this ORF was necessary for repressor activity.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-2203753, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-238937, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-2664716, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-2834335, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-3474623, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-393834, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-4692853, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-6090399, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-6235151, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-6236744, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-6318054, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-6343085, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-6361501, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-6364042, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-6694906, http://linkedlifedata.com/resource/pubmed/commentcorrection/2203754-7108955
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
172
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5477-81
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of Klebsiella aerogenes.
pubmed:affiliation
Department of Biology, University of Michigan, Ann Arbor 48109.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.