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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1990-10-11
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pubmed:abstractText |
Radioimmunoscintigraphy with monoclonal antibodies (MoAbs) to melanoma associated antigens is a new technique that can be used as an additional test to detect ocular melanomas in clinically difficult cases. Immunoscintigraphy with 99mtechnetium-labelled monoclonal antibody fragments of MoAb 225.28S in 14 patients with melanoma yielded a positive image in only six cases (43%). The expression of high molecular weight melanoma-associated antigen (HMW-MAA) was immunohistochemically assessed in melanoma tissue obtained from these 14 patients to find a possible correlation between the results of immunoscintigraphy and expression of the HMW-MAA. The melanoma tissues were immunohistochemically stained by a sensitive immunoperoxidase procedure with three different monoclonal antibodies to the HMW-MAA: 225.28S, Mel-14, and AMF-6. Expression of the antigen detected by MoAb 225.28S was found in 13 of 14 melanomas; the MoAbMel-14 reacted positively with all 14 melanomas; staining with MoAb AMF-6 was achieved in 10 melanomas. No correlation was found between the immunohistochemical staining results, the conventional histopathological findings, and the immunoscintigraphic results. The immunohistochemical staining results suggest that anti-HMW-MAA MoAbs bind to the melanoma tissue and are therefore potentially suitable for immunoscintigraphy.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-1172191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-2500385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-2579631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-2661335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-2804036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-2822079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-2836572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-2917356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-3263596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-3311140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-3608565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-3731128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-374094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-3744725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-6155995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-6165996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-6175965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-6204749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-6376904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-6822741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-6934537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-7033148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203468-7222216
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0007-1161
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
538-41
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:2203468-Aged,
pubmed-meshheading:2203468-Antibodies, Monoclonal,
pubmed-meshheading:2203468-Antigens, Neoplasm,
pubmed-meshheading:2203468-Antigens, Surface,
pubmed-meshheading:2203468-Choroid Neoplasms,
pubmed-meshheading:2203468-Female,
pubmed-meshheading:2203468-Humans,
pubmed-meshheading:2203468-Immunoenzyme Techniques,
pubmed-meshheading:2203468-Male,
pubmed-meshheading:2203468-Melanoma,
pubmed-meshheading:2203468-Melanoma-Specific Antigens,
pubmed-meshheading:2203468-Middle Aged,
pubmed-meshheading:2203468-Neoplasm Proteins,
pubmed-meshheading:2203468-Technetium
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pubmed:year |
1990
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pubmed:articleTitle |
Radioimmunoscintigraphy and immunohistochemistry with melanoma-associated monoclonal antibodies in choroidal melanoma: a comparison of the clinical and immunohistochemical results.
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pubmed:affiliation |
Department of Ophthalmology, University Hospital, Leiden, The Netherlands.
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pubmed:publicationType |
Journal Article,
Comparative Study
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