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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6054
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pubmed:dateCreated |
2011-10-24
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pubmed:databankReference |
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pubmed:abstractText |
The manipulation of protein backbone structure to control interaction and function is a challenge for protein engineering. We integrated computational design with experimental selection for grafting the backbone and side chains of a two-segment HIV gp120 epitope, targeted by the cross-neutralizing antibody b12, onto an unrelated scaffold protein. The final scaffolds bound b12 with high specificity and with affinity similar to that of gp120, and crystallographic analysis of a scaffold bound to b12 revealed high structural mimicry of the gp120-b12 complex structure. The method can be generalized to design other functional proteins through backbone grafting.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1095-9203
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pubmed:author |
pubmed-author:AzoiteiMihai LML,
pubmed-author:BanYih-En AndrewYE,
pubmed-author:CarricoChrisC,
pubmed-author:ChenLeiL,
pubmed-author:CorreiaBruno EBE,
pubmed-author:HuangPo-SsuPS,
pubmed-author:KalyuzhniyOleksandrO,
pubmed-author:KudaJJ,
pubmed-author:KwongPeter DPD,
pubmed-author:McLellanJason SJS,
pubmed-author:SchiefWilliam RWR,
pubmed-author:SchroeterAlexandriaA,
pubmed-author:StrongRoland KRK
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pubmed:issnType |
Electronic
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pubmed:day |
21
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pubmed:volume |
334
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
373-6
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pubmed:meshHeading |
pubmed-meshheading:22021856-Algorithms,
pubmed-meshheading:22021856-Amino Acid Motifs,
pubmed-meshheading:22021856-Amino Acid Sequence,
pubmed-meshheading:22021856-Antibodies, Monoclonal,
pubmed-meshheading:22021856-Antibodies, Neutralizing,
pubmed-meshheading:22021856-Antibody Affinity,
pubmed-meshheading:22021856-Antibody Specificity,
pubmed-meshheading:22021856-Antigens, CD4,
pubmed-meshheading:22021856-Computational Biology,
pubmed-meshheading:22021856-Computer Simulation,
pubmed-meshheading:22021856-Crystallography, X-Ray,
pubmed-meshheading:22021856-Epitopes,
pubmed-meshheading:22021856-HIV Antibodies,
pubmed-meshheading:22021856-HIV Envelope Protein gp120,
pubmed-meshheading:22021856-Models, Molecular,
pubmed-meshheading:22021856-Molecular Mimicry,
pubmed-meshheading:22021856-Molecular Sequence Data,
pubmed-meshheading:22021856-Mutagenesis,
pubmed-meshheading:22021856-Protein Conformation,
pubmed-meshheading:22021856-Protein Engineering,
pubmed-meshheading:22021856-Protein Interaction Domains and Motifs,
pubmed-meshheading:22021856-Surface Plasmon Resonance
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pubmed:year |
2011
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pubmed:articleTitle |
Computation-guided backbone grafting of a discontinuous motif onto a protein scaffold.
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pubmed:affiliation |
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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