Linked Life Data

2201647

Source:http://linkedlifedata.com/resource/pubmed/id/2201647

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1990-9-27
pubmed:abstractText
The importance of superoxide dismutase (SOD) in protecting cells of Nocardia asteroides from the oxidative killing mechanisms within the intact murine host was determined. Murine monoclonal antibodies specific for nocardial SOD and for another nocardial antigen were prepared. Both antibodies adhered to cell surface antigens, as shown by fluorescence-labeled-antibody staining. The anti-nocardial SOD antibody inhibited the effect of nocardial SOD on superoxide generated in vitro. Cells of N. asteroides GUH-2 in log phase of growth were incubated with monoclonal anti-nocardial SOD, another monoclonal antinocardial antibody (not reactive with SOD), or phosphate-buffered saline and then injected intravenously into mice. Total recovery of CFU and inhibition of growth were determined at 3, 24, and 48 h after infection. The brains, kidneys, spleens, lungs, and livers were weighed, homogenized, and plated in order to quantitate the number of organisms in each organ at each time period. There was an initial killing followed by enhanced clearance of N. asteroides from the lungs and livers of mice which had received anti-SOD antibody-treated nocardiae. There was also enhanced early killing in the spleen. At 48 h, there were fewer organisms recovered from the brains, kidneys, and livers of mice which had received anti-SOD antibody-treated nocardia. This was not true for mice which had received antinocardial antibody not specific for SOD. The data demonstrate that surface-associated SOD protects N. asteroides for oxidative killing in vivo during all stages of infection.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2201647-1117067, http://linkedlifedata.com/resource/pubmed/commentcorrection/2201647-166094, http://linkedlifedata.com/resource/pubmed/commentcorrection/2201647-2404874, http://linkedlifedata.com/resource/pubmed/commentcorrection/2201647-352933, http://linkedlifedata.com/resource/pubmed/commentcorrection/2201647-387611, http://linkedlifedata.com/resource/pubmed/commentcorrection/2201647-3880721, http://linkedlifedata.com/resource/pubmed/commentcorrection/2201647-3880799, http://linkedlifedata.com/resource/pubmed/commentcorrection/2201647-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/2201647-5389100, http://linkedlifedata.com/resource/pubmed/commentcorrection/2201647-6336758, http://linkedlifedata.com/resource/pubmed/commentcorrection/2201647-6355320, http://linkedlifedata.com/resource/pubmed/commentcorrection/2201647-6388493, http://linkedlifedata.com/resource/pubmed/commentcorrection/2201647-6777435, http://linkedlifedata.com/resource/pubmed/commentcorrection/2201647-828161
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3122-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Monoclonal antibodies demonstrate that superoxide dismutase contributes to protection of Nocardia asteroides within the intact host.
pubmed:affiliation
Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis 95616.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.