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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
4
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pubmed:dateCreated |
1990-9-27
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pubmed:abstractText |
Replication of HIV-1 requires Tat, which stimulates gene expression through a target sequence, TAR. It is known that TAR is a Tat-responsive target. Since Tat increases transcriptional initiations from the HIV-1 LTR promoter, it is unclear mechanistically how Tat utilizes an RNA target. Here we show that TAR RNA is only one component of the Tat-responsive target. Efficient Tat trans-activation was observed only when TAR was present in conjunction with the HIV-1 LTR NF-kappa B/SP1 DNA sequences. TAR RNA outside of this context produced a suboptimal Tat response. We propose that TAR RNA serves an attachment function directing Tat to the LTR. A Tat protein engineered to interact with LTR DNA could trans-activate through a TAR-independent mechanism. This suggests that Tat also has a DNA target.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0092-8674
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
757-67
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2201451-Animals,
pubmed-meshheading:2201451-Base Sequence,
pubmed-meshheading:2201451-Cell Line,
pubmed-meshheading:2201451-DNA, Viral,
pubmed-meshheading:2201451-DNA Mutational Analysis,
pubmed-meshheading:2201451-Gene Expression Regulation, Viral,
pubmed-meshheading:2201451-Gene Products, tat,
pubmed-meshheading:2201451-HIV-1,
pubmed-meshheading:2201451-HeLa Cells,
pubmed-meshheading:2201451-Humans,
pubmed-meshheading:2201451-Molecular Sequence Data,
pubmed-meshheading:2201451-Promoter Regions, Genetic,
pubmed-meshheading:2201451-RNA, Viral,
pubmed-meshheading:2201451-Recombinant Fusion Proteins,
pubmed-meshheading:2201451-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:2201451-Trans-Activators,
pubmed-meshheading:2201451-Transcription, Genetic,
pubmed-meshheading:2201451-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1990
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|
pubmed:articleTitle |
TAR-independent activation of the HIV-1 LTR: evidence that tat requires specific regions of the promoter.
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pubmed:affiliation |
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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