Linked Life Data

2200944

Source:http://linkedlifedata.com/resource/pubmed/id/2200944

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-9-20
pubmed:abstractText
We propose a methodology for the detection of DNA base changes in restriction enzyme recognition sites. The restriction site mutation (RSM) technique is based upon the detection of DNA sequences resistant to the cutting action of specific restriction enzymes and the amplification of these resistant sequences using the polymerase chain reaction. As outlined, the RSM method has the potential for use in the study of induced base changes in any species, tissue and genes of known DNA sequence for which unique DNA primers are available, and which contains a number of unique restriction enzyme recognition sites.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0267-8357
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
209-12
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Restriction site mutation analysis, a proposed methodology for the detection and study of DNA base changes following mutagen exposure.
pubmed:affiliation
Molecular Biology Research Group, School of Biological Sciences, University College of Swansea, Singleton Park, UK.
pubmed:publicationType
Journal Article