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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-9-12
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pubmed:abstractText |
TRK1, the gene encoding the high affinity K+ transporter in Saccharomyces cerevisiae, is nonessential due to the existence of a functionally independent low affinity transporter. To identify the gene(s) encoding the low affinity K+ transporter, we screened trk1 delta cells for mutants (Kla-) that require higher concentrations of K+ in the medium to support growth. trk1 delta trk2 mutants require up to tenfold higher concentrations of K+ to exhibit normal growth compared to trk1 delta TRK2 cells. K+ and 86Rb+ transport assays demonstrate that the mutant phenotype is due to defective K+ transport (uptake). Each of 38 independent mutants contains a mutation in the same gene, TRK2. Cells deficient for both high and low affinity K+ transport (trk1 delta trk2) exhibit hypersensitivity to low extracellular pH that can be suppressed by high concentrations of K+ but not Na+. TRK1 completely suppresses both the K+ transport defect and low pH hypersensitivity of trk2 cells, suggesting that TRK1 and TRK2 are functionally independent.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2199312-17247336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2199312-2199313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2199312-2648398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2199312-3043197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2199312-356049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2199312-3888294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2199312-3889913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2199312-4359900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2199312-4578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2199312-6144601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2199312-6336730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2199312-6351059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2199312-6384187
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
125
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
305-12
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2199312-Alleles,
pubmed-meshheading:2199312-Biological Transport,
pubmed-meshheading:2199312-Carrier Proteins,
pubmed-meshheading:2199312-Chromosome Mapping,
pubmed-meshheading:2199312-Chromosomes, Fungal,
pubmed-meshheading:2199312-Genes, Fungal,
pubmed-meshheading:2199312-Hydrogen-Ion Concentration,
pubmed-meshheading:2199312-Kinetics,
pubmed-meshheading:2199312-Mutation,
pubmed-meshheading:2199312-Phenotype,
pubmed-meshheading:2199312-Potassium,
pubmed-meshheading:2199312-Rubidium,
pubmed-meshheading:2199312-Saccharomyces cerevisiae
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pubmed:year |
1990
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pubmed:articleTitle |
TRK2 is required for low affinity K+ transport in Saccharomyces cerevisiae.
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pubmed:affiliation |
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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