Linked Life Data

21966575

Source:http://linkedlifedata.com/resource/pubmed/id/21966575

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2011-10-3
pubmed:abstractText
Mammalian spermatozoa become competent for fusion with oocytes while traveling through the female reproductive tract and the oocyte's extracellular investments. Recent studies highlighted the molecular mechanism of the sperm's interactions with the zona pellucida (ZP), the extracellular coat surrounding the oocyte. Fertilizing spermatozoa initiate the sperm acrosome reaction (AR), essential for zona penetration and fusion with the oocyte plasma membrane, before they reach the ZP. However, the exact condition of spermatozoa that leads to successful penetration of the ZP remains unknown. We performed microscopic observations of in vitro fertilization with genetically (EGFP) and chemically (antibody and lectin) labeled spermatozoa to monitor the progression of the AR. Spermatozoa exhibiting EGFP(-)/PNA(+) prior to binding to the ZP initiated zona penetration. This result suggests that spermatozoa that have undergone the AR are still capable of binding and penetrating the ZP.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Jul
pubmed:issn
1942-0889
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
471-6
pubmed:year
2011
pubmed:articleTitle
Video imaging of the sperm acrosome reaction during in vitro fertilization.
pubmed:affiliation
Department of Biological Sciences; Graduate School of Humanities and Sciences; Ochanomizu University; Bunkyo, Tokyo Japan.
pubmed:publicationType
Journal Article