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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1990-8-7
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pubmed:abstractText |
The -10 and -35 regions of E. coli promoter sequences are separated by a spacer region which has a consensus length of 17 base-pairs. This region is thought to contribute to promoter function by correctly positioning the two conserved regions. We have performed a statistical evaluation of 224 spacer sequences and found that spacers which deviate from the 17 base-pair consensus length have nonrandom sequences in their upstream ends. Spacer regions which are shorter than 17 base-pairs in length have a significantly higher than expected frequency of purine-purine and pyrimidine-pyrimidine homo-dinucleotides at the six upstream positions. Spacer regions which are longer than 17 base-pairs in length have a significantly higher than expected frequency of purine-pyrimidine and pyrimidine-purine hetero-dinucleotides at these positions. This suggests that the nature of the purine-pyrimidine sequence at the upstream end of spacer regions affect promoter function in a manner which is related to the spacer length. We examine the spacer sequences as a function of spacer length and discuss some possible explanations for the observed relationship between sequence and length.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-2579077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-2605186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-2647720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-2660103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-2668539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-2942546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-3315462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-3550697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-3882710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-3896120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-3960716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-6206781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-6248238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-6273591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-6278440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-6344016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-6583663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-6754089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-6854650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2194166-6951162
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3597-603
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1990
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pubmed:articleTitle |
E. coli promoter spacer regions contain nonrandom sequences which correlate to spacer length.
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pubmed:affiliation |
Program in Molecular Biology, University of Wisconsin, Madison 53706.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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