Linked Life Data

2190925

Source:http://linkedlifedata.com/resource/pubmed/id/2190925

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6 Pt 2
pubmed:dateCreated
1990-7-13
pubmed:abstractText
We developed six kinds of monoclonal antibodies against endothelin (ET)-1 recognizing different epitopes with high affinities (5 x 10(10) M-1 to 5 x 10(11) M-1). Using these monoclonal antibodies, we developed radioimmunoassays for ET-1 with different specificities. Cross-reactivities with ET-2 ranged from 80% to 100%, and those with ET-3 ranged from 3% to 60%. Patients with essential hypertension (n = 20) showed a significant elevation in the plasma ET-1-LI level compared with age-matched control subjects (n = 12) (30.1 +/- 1.4 pg/ml versus 18.5 +/- 0.9 pg/ml, p less than 0.01). The plasma ET-1-LI level in hypertensive patients in stages II and III (World Health Organization classification) was significantly higher than that in those patients in stage I. There was no significant correlation between the plasma ET-1-LI level and systolic blood pressure (r = 0.11), diastolic blood pressure (r = -0.13), or age (r = 0.24) in all patients studied who had essential hypertension. In the neutralization experiment, monoclonal antibodies attenuated ET-1-induced contraction of rat aortic rings and the pressor action of ET-1 in pithed rats in vivo. The present study demonstrates the elevated plasma ET-1-LI level in patients with essential hypertension. Monoclonal antibodies developed in this study can become powerful tools to investigate the pathophysiological significance of ET in essential hypertension.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0194-911X
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
734-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Application of monoclonal antibodies for endothelin to hypertensive research.
pubmed:affiliation
Department of Medicine, Kyoto University School of Medicine, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't