Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1979-6-29
|
| pubmed:abstractText |
Reports by a number of investigators have described the thymus-derived (T)-cell dependence of immunoglobulin synthesis by pokeweed mitogen (PWM) stimulated human peripheral blood bone marrow-derived (B) cells. Because of the cooperative nature of this in vitro system, it was chosen for examination of the differential effects of low density lipoprotein inhibitor (LDL-In) on B- and T-cell functions. Supernates from 7-d cultures that contained either peripheral blood mononuclear cells (PBM) or combinations of isolated lymphocyte populations were assayed for immunoglobulin (Ig)G by competitive inhibition radio-immunoassay. LDL-In suppression of whole PBM IgG synthesis occurred at 5-20 mug protein/ml and was independent of PWM concentration. Maximal suppression required preincubation of cells with LDL-In before stimulation. Suppression was also observed when B cells alone were exposed for 24 h to LDL-In before PWM stimulation; these suppressed B cells were not rescued by normal T cells. Exposure of T cells alone to low doses of LDL-In for 24 h augmented, but high doses suppressed, IgG synthesis, suggesting a differential effect on T-helper vs T-suppressor cell populations. Independent LDL-In exposure of T-helper or T-suppressor cell enriched populations, separated by rosetting with IgG- or IgM-coated ox erythrocytes, identified the T-suppressor cell populations as the most sensitive of the lymphocyte populations tested. The sensitivities of lymphocyte subpopulations to LDL-In, relative to PBM, were 2.8, 1.2, and 0.3 for the T-suppressor cells, B cells and T-helper cells, respectively. Thus, both B and T lymphocytes are sensitive to and can be regulated by LDL-In. In addition, the biologic activity observed when unseparated PBM are exposed to LDL-In appears to represent a composite of the sensitivity of each of the lymphocyte subpopulations.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-1083396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-1086217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-1086250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-17835063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-178796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-190318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-193990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-207735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-300089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-300495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-301544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-302278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-305449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-328777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-336837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-342605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-4107365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-4818458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-792339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-792341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-794413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219033-800393
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0021-9738
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
63
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
193-201
|
| pubmed:dateRevised |
2010-9-2
|
| pubmed:meshHeading | |
| pubmed:year |
1979
|
| pubmed:articleTitle |
Differential sensitivity of lymphocyte subpopulations to suppression by low density lipoprotein inhibitor, an immunoregulatory human serum low density lipoprotein.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
|