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| pubmed-article:2188952 | lifeskim:mentions | umls-concept:C0035541 | lld:lifeskim |
| pubmed-article:2188952 | lifeskim:mentions | umls-concept:C1442161 | lld:lifeskim |
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| pubmed-article:2188952 | lifeskim:mentions | umls-concept:C1524062 | lld:lifeskim |
| pubmed-article:2188952 | lifeskim:mentions | umls-concept:C1704711 | lld:lifeskim |
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| pubmed-article:2188952 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
| pubmed-article:2188952 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:2188952 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:2188952 | pubmed:dateCreated | 1990-7-3 | lld:pubmed |
| pubmed-article:2188952 | pubmed:abstractText | The cloning and overexpression of the Escherichia coli rna gene encoding RNase I are described. Only a single copy of the rna gene is present on the E. coli chromosome. Although cells with as much as a 100-fold increase in RNase I activity were constructed, little effect on cell growth was observed. Overexpressed RNase I was found in the periplasmic space to the same degree (approximately 85%) as wild-type enzyme, suggesting no limitation in RNase I transport. The rna clone was used to identify a deletion strain totally lacking the rna gene. The normal growth of this strain showed that RNase I is not essential for cell viability. Extracts from the RNase I deletion strain still retained a low level of RNase activity in the presence of EDTA, conclusively demonstrating the existence of additional EDTA-active RNases in E. coli. The possibility of a RNase I inhibitor is also discussed. | lld:pubmed |
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| pubmed-article:2188952 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:2188952 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2188952 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2188952 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:2188952 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:2188952 | pubmed:author | pubmed-author:DeutscherM... | lld:pubmed |
| pubmed-article:2188952 | pubmed:author | pubmed-author:ZhuL QLQ | lld:pubmed |
| pubmed-article:2188952 | pubmed:author | pubmed-author:PadmanabhaK... | lld:pubmed |
| pubmed-article:2188952 | pubmed:author | pubmed-author:GangopadhyayT... | lld:pubmed |
| pubmed-article:2188952 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2188952 | pubmed:volume | 172 | lld:pubmed |
| pubmed-article:2188952 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2188952 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2188952 | pubmed:pagination | 3146-51 | lld:pubmed |
| pubmed-article:2188952 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2188952 | pubmed:meshHeading | pubmed-meshheading:2188952-... | lld:pubmed |
| pubmed-article:2188952 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2188952 | pubmed:articleTitle | Escherichia coli rna gene encoding RNase I: cloning, overexpression, subcellular distribution of the enzyme, and use of an rna deletion to identify additional RNases. | lld:pubmed |
| pubmed-article:2188952 | pubmed:affiliation | Department of Biochemistry, University of Connecticut Health Center, Farmington 06032. | lld:pubmed |
| pubmed-article:2188952 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2188952 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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