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rdf:type |
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lifeskim:mentions |
umls-concept:C0009015,
umls-concept:C0014442,
umls-concept:C0014834,
umls-concept:C0017337,
umls-concept:C0035541,
umls-concept:C0679058,
umls-concept:C1442161,
umls-concept:C1514559,
umls-concept:C1524062,
umls-concept:C1524063,
umls-concept:C1547699,
umls-concept:C1704711,
umls-concept:C2700640
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pubmed:issue |
6
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pubmed:dateCreated |
1990-7-3
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pubmed:abstractText |
The cloning and overexpression of the Escherichia coli rna gene encoding RNase I are described. Only a single copy of the rna gene is present on the E. coli chromosome. Although cells with as much as a 100-fold increase in RNase I activity were constructed, little effect on cell growth was observed. Overexpressed RNase I was found in the periplasmic space to the same degree (approximately 85%) as wild-type enzyme, suggesting no limitation in RNase I transport. The rna clone was used to identify a deletion strain totally lacking the rna gene. The normal growth of this strain showed that RNase I is not essential for cell viability. Extracts from the RNase I deletion strain still retained a low level of RNase activity in the presence of EDTA, conclusively demonstrating the existence of additional EDTA-active RNases in E. coli. The possibility of a RNase I inhibitor is also discussed.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-13539038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-13820107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-14257624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-2413440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-2580634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-2653829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-2676990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-2828310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-3311729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-370414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-4358625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-4563069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-4570595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-4860493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-4892383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-5320389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-5331244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-6187365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-6207170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-6248430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-6260575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188952-7007341
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
172
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3146-51
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1990
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pubmed:articleTitle |
Escherichia coli rna gene encoding RNase I: cloning, overexpression, subcellular distribution of the enzyme, and use of an rna deletion to identify additional RNases.
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pubmed:affiliation |
Department of Biochemistry, University of Connecticut Health Center, Farmington 06032.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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