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rdf:type |
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lifeskim:mentions |
umls-concept:C0007595,
umls-concept:C0014239,
umls-concept:C0017337,
umls-concept:C0033684,
umls-concept:C0043393,
umls-concept:C0105810,
umls-concept:C0220781,
umls-concept:C0332148,
umls-concept:C1143198,
umls-concept:C1268443,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C1883254,
umls-concept:C2700640
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pubmed:issue |
6
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pubmed:dateCreated |
1990-6-25
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pubmed:databankReference |
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pubmed:abstractText |
Yeast kre mutants define a pathway of cell wall (1----6)-beta-D-glucan synthesis, and mutants in genes KRE5 and KRE6 appear to interact early in such a pathway. We have cloned KRE5, and the sequence predicts the product to be a large, hydrophilic, secretory glycoprotein which contains the COOH-terminal endoplasmic reticulum retention signal, HDEL. Deletion of the KRE5 gene resulted in cells with aberrant morphology and extremely compromised growth. Suppressors to the KRE5 deletions arose at a frequency of 1 in 10(7) to 1 in 10(8) and permitted an analysis of deletions which were found to contain no alkali-insoluble (1----6)-beta-D-glucan. These results indicate a role for (1----6)-beta-D-glucan in normal cell growth and suggest a model for sequential assembly of (1----6)-beta-D-glucan in the yeast secretory pathway.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-13121015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-2661018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-2668738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-2942941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-3038686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-3049074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-3327750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-344137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-348280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-349166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-3896128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-3907857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-3916769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-391805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-3991809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-4359920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-4590991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-6246361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-6259625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-6300031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-6310324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-6371496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-6394718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-6423828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-7050670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188106-778330
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3013-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2188106-Amino Acid Sequence,
pubmed-meshheading:2188106-Base Sequence,
pubmed-meshheading:2188106-Cell Wall,
pubmed-meshheading:2188106-Chromosome Deletion,
pubmed-meshheading:2188106-Cloning, Molecular,
pubmed-meshheading:2188106-DNA, Fungal,
pubmed-meshheading:2188106-Endoplasmic Reticulum,
pubmed-meshheading:2188106-Escherichia coli,
pubmed-meshheading:2188106-Fungal Proteins,
pubmed-meshheading:2188106-Genes, Fungal,
pubmed-meshheading:2188106-Genotype,
pubmed-meshheading:2188106-Glucans,
pubmed-meshheading:2188106-Glycoproteins,
pubmed-meshheading:2188106-Molecular Sequence Data,
pubmed-meshheading:2188106-Mutation,
pubmed-meshheading:2188106-Plasmids,
pubmed-meshheading:2188106-Restriction Mapping,
pubmed-meshheading:2188106-Saccharomyces cerevisiae,
pubmed-meshheading:2188106-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:2188106-beta-Glucans
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pubmed:year |
1990
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pubmed:articleTitle |
The yeast KRE5 gene encodes a probable endoplasmic reticulum protein required for (1----6)-beta-D-glucan synthesis and normal cell growth.
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pubmed:affiliation |
Department of Biology, McGill University, Montreal, Quebec, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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