Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1990-6-25
pubmed:abstractText
The mammalian c-fps/fes proto-oncogene encodes a 92-kilodalton cytoplasmic protein-tyrosine kinase (p92c-fes), which is expressed in immature and differentiated hematopoietic cells of the myeloid lineage. To determine the limits of the c-fps/fes locus and to investigate the cis-acting sequences required to direct appropriate tissue-specific expression, a 13-kilobase-pair fragment of human genomic DNA containing the entire c-fps/fes coding sequence was introduced into the mouse germ line. Transcription of the human c-fps/fes transgene was highest in bone marrow and showed a tissue distribution identical to that of the endogenous mouse gene. Macrophages cultured from transgenic mouse bone marrow contained particularly high levels of human and murine c-fps/fes RNA. Furthermore, expression of human c-fps/fes RNA induced a proportionate increase in the level of the p92c-fes protein-tyrosine kinase in bone marrow, bone marrow-derived macrophages, and spleen. Elevated levels of normal human p92c-fes had no obvious effect on mouse development or hematopoiesis. Remarkably, given the short 5'- and 3'-flanking sequences, expression of the human proto-oncogene in bone marrow was independent of integration site, was proportional to the transgene copy number, and was of comparable efficiency to that of the endogenous mouse c-fps/fes gene. The 13-kilobase-pair fragment therefore defines a genetic locus sufficient for the appropriate tissue-specific expression of the fps/fes protein-tyrosine kinase and includes a dominant cis-acting element that directs integration-independent myeloid expression in transgenic mice.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2408759, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2426571, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2457811, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2458842, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2555699, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2564317, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2567000, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2683070, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2922063, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2924351, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2924354, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2986115, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2987674, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-2990723, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-3021984, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-3023061, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-3031090, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-3060793, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-3111720, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-3119227, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-3329718, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-3352601, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-3480506, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-3488499, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-3594565, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-3690667, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-4065096, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-6281890, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-6538699, http://linkedlifedata.com/resource/pubmed/commentcorrection/2188092-7269249
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2521-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Myeloid expression of the human c-fps/fes proto-oncogene in transgenic mice.
pubmed:affiliation
Division of Molecular and Developmental Biology, Mount Sinai Hospital Research Institute, Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't