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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1990-6-19
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pubmed:abstractText |
An integration plasmid, pIARL28, containing the ribosomal DNA gene as a homologous recombination sequence was constructed for introduction of the alpha-acetolactate decarboxylase gene into brewer's yeast. The transformation efficiency of pIARL28 was 20- to 50-fold higher than those of the other YIp vectors, as yeast cells had approximately 140 copies of the ribosomal DNA gene. All transformants showed very high alpha-acetolactate decarboxylase activity due to the multiple integrated copies of the plasmid. The transformants were grown in nonselective conditions, and segregants which had maintained the alpha-acetolactate decarboxylase expression cassette but no other vector sequences were isolated. Southern analysis showed that these marker-excised segregants contained more than 20 copies of the alpha-acetolactate decarboxylase gene and were stably maintained under nonselective conditions. Fermentation tests confirmed that the diacetyl concentration was considerably reduced in wort fermented by these marker-excised segregants. The degree of reduction was related to the copy number of the alpha-acetolactate decarboxylase gene.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2187408-2827308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2187408-3015730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2187408-3278689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2187408-334774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2187408-375221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2187408-388424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2187408-3905514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2187408-394173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2187408-5365012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2187408-6310321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2187408-6345791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2187408-6369254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2187408-6987539
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
997-1003
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2187408-Carboxy-Lyases,
pubmed-meshheading:2187408-DNA, Fungal,
pubmed-meshheading:2187408-DNA, Ribosomal,
pubmed-meshheading:2187408-Diacetyl,
pubmed-meshheading:2187408-Fermentation,
pubmed-meshheading:2187408-Genes, Fungal,
pubmed-meshheading:2187408-Genetic Vectors,
pubmed-meshheading:2187408-Plasmids,
pubmed-meshheading:2187408-Saccharomyces cerevisiae,
pubmed-meshheading:2187408-Transformation, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
Application of a ribosomal DNA integration vector in the construction of a brewer's yeast having alpha-acetolactate decarboxylase activity.
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pubmed:affiliation |
Central Laboratories of Key Technology, Kirin Brewery Co., Ltd., Gunma, Japan.
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pubmed:publicationType |
Journal Article
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