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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2011-8-23
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pubmed:abstractText |
Regulation of B cell receptor (BCR)-induced Ca(2+) signaling by CD40 co-stimulation was compared in long-term BCR-stimulated immature (WEHI-231) and mature (Bal-17) B cells. In response to long-term pre-stimulation of immature WEHI-231 cells to ?-IgM antibody (0.5~48 hr), the initial transient decrease in BCR-induced [Ca(2+)](i) was followed by spontaneous recovery to control level within 24 hr. The recovery of Ca(2+) signaling in WEHI-231 cells was not due to restoration of internalized receptor but instead to an increase in the levels of PLC?2 and IP(3)R-3. CD40 co-stimulation of WEHI-231 cells prevented BCR-induced cell cycle arrest and apoptosis, and it strongly inhibited the recovery of BCR-induced Ca(2+) signaling. CD40 co-stimulation also enhanced BCR internalization and reduced expression of PLC?2 and IP(3)R-3. Pre-treatment of WEHI-231 cells with the antioxidant N-acetyl-L-cysteine (NAC) strongly inhibited CD40-mediated prevention of the recovery of Ca(2+) signaling. In contrast to immature WEHI-231 cells, identical long-term ?-IgM pre-stimulation of mature Bal-17 cells abolished the increase in BCR-induced [Ca(2+)](i), regardless of CD40 co-stimulation. These results suggest that CD40-mediated signaling prevents antigen-induced cell cycle arrest and apoptosis of immature B cells through inhibition of sustained BCR-induced Ca(2+) signaling.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21860597-10048784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21860597-10430908,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/21860597-18625201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21860597-21461234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21860597-3520839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21860597-3680375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21860597-8662540
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pubmed:language |
eng
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pubmed:journal |
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pubmed:status |
PubMed-not-MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1226-4512
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
179-87
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pubmed:year |
2011
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pubmed:articleTitle |
CD40 Co-stimulation Inhibits Sustained BCR-induced Ca Signaling in Response to Long-term Antigenic Stimulation of Immature B Cells.
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pubmed:affiliation |
Department of Physiology, SBRI, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea.
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pubmed:publicationType |
Journal Article
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