Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-6-7
pubmed:abstractText
The role of insulin in modulating phosphoinositide breakdown and accumulation of inositol phosphates was investigated in isolated rat pancreatic islets by using GPAIS (guinea-pig anti-insulin antiserum) that neutralizes effects of insulin in the medium. At either 3.0 mM- or 16.7 mM-glucose or 3.0 mM-glucose plus 10 microM-arecaidine propargyl ester (muscarinic receptor agonist), GPAIS (but not control serum) was able to increase InsP2 and InsP3, but not InsP, in myo-[3H] inositol-prelabelled islets. The effect of GPAIS on 3H incorporation into InsP3 was dose-dependent, with a half-maximal effect at a concentration able to bind 4004 +/- 163 microunits of insulin. A specific mass assay of the biologically relevant isomer Ins (1,4,5)P3 revealed a huge increase (greater than 3-folf). Formation of PtdIns, PtdInsP and PtdInsP2 was not affected by GPAIS. This is indirect evidence for an effect of insulin on inositide metabolism, and therefore endogenously released insulin may have led to an underestimation in earlier studies of effects of insulinotropic substances on inositol phosphate accumulation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-1107025, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-11990, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-14258509, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-215685, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-3024737, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-3028890, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-385305, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-4084229, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-4740217, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-5333500, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-6092036, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-6095092, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-6131819, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-6146314, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-6275838, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-6309146, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-6316930, http://linkedlifedata.com/resource/pubmed/commentcorrection/2185741-6988459
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
267
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
339-42
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Anti-insulin antiserum increases inositol phosphate accumulation in rat pancreatic islets.
pubmed:affiliation
Department of Pharmacology, University of Tübingen, Federal Republic of Germany.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't