Linked Life Data

2185019

Source:http://linkedlifedata.com/resource/pubmed/id/2185019

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-6-6
pubmed:abstractText
Oxygen-evolving photosystem II core complexes were prepared from spinach by solubilizing photosystem II membrane fragments with dodecyl-beta-D-maltoside. The core complexes consist of the intrinsic 47-kDa, 43-kDa, D1 and D2 polypeptides, the two subunits of cytochrome b559 and the extrinsic 33-kDa protein. In the presence of 50 mM CaCl2 they exhibit a high oxygen evolution rate of 1.3 +/- 0.2 mmol O2.mg chlorophyll-1.h-1 with either 2,6-dichloro-benzoquinone or K3[Fe(CN)6] as acceptor. Electron micrographs of these complexes reveal an obtuse triangular structure in when viewed from the top measuring 15.3 nm on one side and 10.6 nm on the other two sides. An average height of 7.3 nm was determined from the side view position. These data are in good agreement with previously reported dimensions for photosystem II core complexes [Irrgang, K.-D., Boekema, E. J., Vater, J. and Renger, G. (1988) Eur. J. Biochem. 178, 209-217]. In contrast to previous reports the extrinsic 33-kDa subunit could be resolved for the first time. It appears as a small protrusion when the complex is viewed from the side and seems to cover the lumenal side of the core complex appearing as a disk with a thickness of 1.5-3.3 nm.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
189
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-53
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Functional and structural analysis of photosystem II core complexes from spinach with high oxygen evolution capacity.
pubmed:affiliation
Max-Volmer-Institut für Biophysikalische und Physikalische Chemie, Technische Universität Berlin.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't